put (card field F1 + card field F2 + card field F3 + card field F4 + card field F5 + card field F6 + card field F8) into card field F9
set the numberFormat to "00.00"
put ((card field F3 + card field F4) / card field F9) * 100 into card field F10
put ((card field F5 + card field F6)/ card field F9) * 100 into card field F11
end mouseUp
-- part 13 (field)
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-- part name: F9
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-- part name: cover
-- part 18 (button)
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-- part name: NEXT
----- HyperTalk script -----
on mouseUp
go to next card
end mouseUp
-- part 19 (button)
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-- part name: PREV.
----- HyperTalk script -----
on mouseUp
go back
end mouseUp
-- part 20 (field)
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-- part name: coverone
-- part contents for background part 1
----- text -----
GENE MAPPING
-- part contents for background part 2
----- text -----
On the right you will see a typical, three-point mapping exercise using the material discussed in the previous card. Click on the ACTIVATE button and let the program run to completion. It will take around 4 minutes to run the program. When
"DONE" appears in the lower right hand corner you can analyze your data. Note that the parental classes are represented with the largest number of progeny [ either wild or
(+) for all of the recessives in question or homozygous for all three recessives]. In addition to these classes, there are additional phenotypes that represent crossover classes. When counting from the top, boxes 3 and 4 represent crossing over between [ colorless and (shrunken, waxy)]. Boxes 5 and 6 represent crossovers between [ waxy and
(colorless, shrunken)]. The lowest frequency of crossing over occurs between [shrunken and (colorless, waxy)]. Generally the very low frequencies of crossing over occur in the double crossover classes. We can consider boxes 7 & 8 then to be such classes. Generally the values contained in the double crossover classes are too small to affect the overall calculation of gene loci. However, in very careful work, they must be considered. Since it is a double crossover class, then that data must be added to each of the single crossover glasses in the upper boxes. If you have not already done so, click on the ANALYZE button and let the program determine the percent of crossing over between the genes in question. Ok, now you can continue with the mapping exercise.
The calculated percentages of crossing over between [ colorless
(shrunken, waxy)] depicted as
{c/wx,ch} and between [waxy
(colorless, shrunken)] depicted as
{wx/c,sh} can be taken as the percentage of the chromosome length separating these genes. However the exact arrangement is not quite certain. It could be one of two possibilities.
1. wx_________________c__sh or
2. wx_________________sh__c
If option #1 were the true arrangement of the genes on the chromosome, then for colorless to separate from waxy and shrunken, a double crossover event would have to occur. However a glance at the data on the right shows a double crossover class involving the separation of shrunken from colorless, waxy. This rare event occurred much less often than the separation of colorless from the shrunken, waxy genes. Hence the actual arrangement of the genes along the length of the chromosome is shown by option #2. Note that it is possible to activate the program several times to get a better "fix" on the actual distances separating the loci. To do this, do not clear the data from the boxes when you activate the program a second time.
